MPprimer: a program for reliable multiplex PCR primer design
نویسندگان
چکیده
منابع مشابه
Development of a Primer Design Program for Multiplex PCR
Multiplex PCR method is a powerful technology as a potent tool to amplify specific DNA fragments from genomic DNA using Taq DNA polymerase and primer sets. Since multiple reactions occur in single tube, multiplex PCR has the potential to produce considerable savings of time and effort. Its applications are widely ranged from in vitro cloning of specific regions of DNA to diagnosis of disease [1...
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The analysis of DNA methylation at CpG dinucleotides has become a major research focus due to its regulatory role in numerous biological processes, but the requisite need for assays which amplify bisulfite-converted DNA represents a major bottleneck due to the unique design constraints imposed on bisulfite-PCR primers. Moreover, a review of the literature indicated no available software solutio...
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Kariyama and colleagues (5) recently described a multiplex PCR method for the detection of the vanA, vanB, vanC1, and vanC2/C3 genes, in addition to primers for the detection of the ddl genes of Enterococcus faecalis and E. faecium and of 16S rRNA (PCR control). However, the vanB primers used by these authors were derived from the sequence of the vanB1 subtype (1) and are incapable of detecting...
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ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2010
ISSN: 1471-2105
DOI: 10.1186/1471-2105-11-143